Hyoscyamus muticus hairy root culture pdf

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Description du sujet. Description of the subject. The hyoscyamine, a tropane alkaloid, widely used in medicine, can be produced from Datura sp.

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The results presented in this chapter are mostly based on our own work with Hyoscyamus muticus carried out during the years — in our laboratories in Finland and Italy. Unable to display preview. Download preview PDF.

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Hairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop Agastache foeniculum via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes A4, A7 andwere used for induction of hairy roots in four various explants hypocotyl, cotyledon, one-month-old leaf and five-month-old leaf of Anise hyssop.

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The latter gave the highest anisodamine content 0. Both bioreactors gave similar productivities of scopolamine 1 and 0. Tropane alkaloids belong to widely used drugs of plant origin.

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Plants remain a major source of new drugs, leads and fine chemicals. Cell cultures deriving from plants offer a fascinating tool to study plant metabolic pathways and offer large scale production systems for valuable compounds — commercial examples include compounds such as paclitaxel. The major constraint with undifferentiated cell cultures is that they are generally considered to be genetically unstable and cultured cells tend to produce low yields of secondary metabolites especially over time.

The aim of this study was to use the, hairy root system for increasing the scopolamine content in Atropa belladonna. Agrobacterium rhizogenes ATCC was utilized to produce hairy roots. The culture was carried out in a 1.

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Bos R. Analytical aspects of phytotherapeutic valerian preparations. Phytochemical Analysis, 7: DOI:

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Search in: All Webpages Books Journals. Tarbiat Modares University Press. Journal of Agricultural Science and Technology.


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